Category Archives: Skills

A detailed description for all of my skills

Ethanol Reformation to Hydrogen

01/25/2016 Eric Hanson Leave a comment

When I was working at the Environment and Energy Research Center (EERC) on the hydrogen fuel cell feasibility study, I was given the opportunity to make a few extra bucks helping out a lab scientist. He was studying different catalyst materials to see how efficiently they would reform ethanol into hydrogen.

My primary function was data entry, but I was present for much of the testing. When I was working with him, we used platinum or a platinum alloy. We setup the experiment and recorded the amount of hydrogen and catalyst weight loss at 15 – 30 minute intervals.

One day while we were running the experiment and chatting about places to eat around Grand Forks, our conversation started to drift around and we were laughing a lot and having a really pleasant time. For a time that was great and then we started feeling a bit boozed up, and then we realized it. We had an ethanol leak. We had to evacuate the lab after finding out we had a defective stopper on the ethanol bottle.

Skills

DNA Extraction Assay

01/24/2016 Eric Hanson Leave a comment

At MSUM I participated in undergraduate research each semester. This was a great opportunity to do some independent work in the lab and receive credits for working on a project with a professor.

One of the semesters I worked with another student running DNA extraction assays on liverworts. From what I remember, we cleaned the liverworts well, processed the plant material down and then centrifuged with silica beads. I don’t remember the process exactly, but we probably used a kit similar to this one.

We also amplified the DNA we extracted using PCR.

Skills

Cytology Specimens for Theragnostic Cancer Treatment

01/23/2016 Eric Hanson Leave a comment

I was always looking at learning how to do new techniques and operate new machinery while I was working at Caris Lifesciences. The projects that take you out of the repetitive daily tasks were what I lived for. Cytology was a great skill to learn.

I was approached about how I would feel about learning how to process fluid specimens during grossing, or cytology. Since this was something new, I wanted to see how it went. We were going to receive all types of bodily fluids, most of them containing blood, but not necessarily always.

The process wasn’t super complicated, but there were a lot more manual steps involved than simply putting tissue in a tissue processor.

It involved pipetting the blood into smaller vials. These vials were then placed in a centrifuge, spun down, and had the supernatant pipetted off the top, and then spun down more to form a pellet.

cytology — A bloody fluid sample on its way to becoming a pellet in a centrifuge tube

The idea was to get as much of the fluid out as possible. This process continued until a satisfactory pellet was made.

The pellets were then combined and heated agarose gel was added to the cell pellet. The agarose infused cells were then wrapped in lens paper and stuck in the tissue processor to further desiccate the sample. After tissue processing, it was embedded in paraffin and cut on a microtome like any other sample.

With all the steps, these samples could take anywhere from 45 minutes to a few hours depending on the quality of the fluid being sent in. If it wasn’t fresh, it was hard, and if it had formalin in it, more centrifuging was required.

I was the only person, besides the lab director, who was responsible for doing these samples. It was a responsibility I gladly accepted, plus if one of these cytology samples came in on a weekend (which I volunteered to work), I would have to process them anyway. I did so with little supervision or training, but just followed the assay and everything came out well each time. Most of the specimens I handled yielded good molecular data later down the process line.

Skills

Anti-Body Dilution Preparation

01/22/2016 Eric Hanson Leave a comment

Diluting a solution is pretty easy. Usually you are taking a stock solution and adding a buffer or other solution to it to get the required dilution. The math is rather simple here as well.

C_1\times V_1 = C_2\times V_2

Where:

C_1 =

Initial concentration or molarity.

V_1 =

Initial volume.

C_2 =

Final concentration or molarity.

V_2 =

Final volume

If math isn’t your strong suit, you can always use an online calculator to find volume of solution you need for your dilution.

When doing anti-body dilutions for IHC tests, everything is measure in micro liters (μL) and a micro-pipette is used to dispense the required volumes. Some common mistakes can occur such as, leaving out the antibody and allowing it to reach room temperature or not stirring/mixing the antibody before doing the dilution. Sometimes the antibody can settle/precipitate, so mixing should also occur once diluted and before being placed on an instrument.

Skills

Water Sampling Techniques

01/22/2016 Eric Hanson Leave a comment

Physio-chemical water analysis can be done many different ways. Water can be collected and sent off to a laboratory, or you can collect the data with hand held probes. If you require a more in-depth analysis it’s always a good idea to have it sent to a lab that specializes in water quality analysis, but if you are only doing a comparative study, it doesn’t make sense to spend all the money getting a professional lab to analyse the water quality.

While I was attending Minnesota State University: Moorhead (MSUM), we used may hand held probes on our assignments and student projects. We did a study on crayfish looking for preferred substrate size, and while doing that we recorded some simple water measurements such as: temperature, turbidity, and dissolved oxygen.

When working the life history study of the Mohave Tui Chub, we took many more readings. Temperature was taken on several sites due to the varying depth of the water. We wanted to make sure that the temperature did not interfere with the interaction between the chub and the mosquito fish. We also sampled dissolved oxygen, salinity, pH, and turbidity. Most of the measurements were made using a handheld probe. Some of the probes were multi functional, but some were uni-taskers. We took recordings several times per week at multiple points in the lake and ponds.

We also collected water samples in glass vials and delivered them to a professional water analysis laboratory in Los Angeles.

Skills

West River Waterfowl Surveys

01/21/2016 Eric Hanson Leave a comment

I worked for two successive summers at the HaPET office in the Bismarck, ND USFWS building. We were concerned with areas in North Dakota and South Dakota that were west of the Missouri river, or west river.

This project started as an offshoot of the Partners for Fish and Wildlife (PFW) program. This program provided help for landowners who had a lack of water on their property. The USFWS helped them build dams in water basins for irrigation and livestock.

Each year, before we started the survey work, we would directly call each land owner to make sure they would still allow us on their property. According to the agreement the landowner signed, we were allowed unobstructed access to the wetlands that were created, but we didn’t want to ruffle any feathers. Most folks were quite happy to have our help and would invite me in for coffee so we could talk about their land.

Then it became time to pound the pavement and get to all the wetlands. The surveys usually didn’t take too much time, but some were very hard to access. We were given an ATV, binoculars, and a spotting scope. The best way to survey the waterfowl were when they were on the water and not moving a lot. So sneaking up on them, or scoping them from a distance were the best methods.

Typical wetland, with a lot of waterfowl on it

These were similar to the four-square-mile surveys. I really enjoyed these surveys. I had to travel to the southern most parts of South Dakota, part of Montana, to the northern most parts of North Dakota. Every day was different, and I really enjoyed that. Some times we would be laid up at a hotel during inclement weather, but typically we could work through most weather conditions.

Green-wing teal male in full breeding plumage

We went out once in the spring to each wetland, and then again towards the fall to do a brood survey. The brood survey is super hard because all the adults have lost their breeding plumage, and the young hatch year birds, looked very similar to each other. It was much easier to ID them if the adults were around.

Late season plumage, it starts to get ratty looking before they molt their feathers

Skills

Zooplankton Counting and Classification

01/20/2016 Eric Hanson Leave a comment

My primary function while participating in the life history study of the Mohave Tui Chub was zooplankton counting. This was the first year of the field project, with several more years of study scheduled. During this phase of the project, we were developing a deeper understanding of the environment the chub lived in. We also did extensive water sampling and created micro habitats called mescosms. If you would like to read the dessertation paper Sujan Henkanaththegedara wrote about the Mohave Tui Chub, please see it here.

zooplankton1 — Conochilidae family – Rotifers

We collected zooplankton utilizing the horizontal water sampler which allowed us to get a representative sample at various depths across the waters were were testing. Once the water sample was collected, the invertebrates were strained out and placed in a sugar-formalin solution. Sugar formalin was chosen due to it’s similar osmostic characteristics to the saline environment they were taken out of.

zooplanktoncounts — Zooplankton counting station. The pink vials contain sugar-formalin and lots of invertebrates

The samples were than aliquoted out and a several mL sample was dumped in a zooplankton counting wheel. The wheel consists of a non-continuous circular line inset in a clear plastic dish. It is then placed under a dissecting scope and you start counting and classifying the zooplankton as you go around the wheel. We did this four times for each sample.

When we found an interesting or unknown specimen, we would take it over to a compound microscope on a glass slide. This allowed for much greater magnification so we could further classify the zooplankton.

For the study, we were not interested in classifying individuals down to the species, but instead got them all down to the family. Most of the samples contained many rotifers, and even on occasion we found some of the aquatic invertebrates such as water mites and chironomidae spp.

These photographs were taken through a compound microscope on a glass slide. I love using the Image-Based Key to the Zooplankton of North America, and recommend it’s use if you ever need to get down to the family of zooplankton you’re looking at. After that, a comprehensive key can be used to get to the species, but you’ll want to have several individuals for dissection, and a really good microscope.

Skills

Forb and Graminoid Identification

01/19/2016 Eric Hanson Leave a comment

While doing belt transects, this skill was essential to the research project. Without knowing what all of the grasses and leafy plants were, how could you determine if it was a native plant or not?

During the first month of employment on this project, the members of the team all went to local herbariums and studied identification keys so we could accurately identify all the foliage we came across.

Learning this skills was the most rewarding experience I’ve had at a job. The task was daunting in the amount of things you had to know. When I was unsure of something, I would take a picture from every single angle I could, or even dig out the plant by the roots so I could later identify what it was. This lead to many plant pressings and a huge amount of pictures. I was even working on a photographic guide to the wild flowers and also a guide to ligules. Ligules are used to identify grasses before the seed head has had a chance to grow. It’s the only way to determine which species a grass is in the springtime, even for cool season grass.

I did this for 9 hours a day for 5 months and learned an immense amount of information. I wish I could have retained it all. Some random images from the collection are below.

Key to North Dakota Grasses and good short photographic guide to forbs of North Dakota. The later includes a lot of very common plants to the North Dakota prairie. Another short photographic guide is located here.

061208 Weld county - Weber WPA — Geum triflorum-Prairie Smoke

061208 Weld county - Weber WPA_1 — Basal foliage of G. triflorum

061208 Weld county - Weber WPA_2 — Lithospermum canescens – Hoary Puccoon

061208 Weld county - Weber WPA_7 — Cynoglossum officinale – Houndstongue

061208 Weld county - Weber WPA_8 — Elaeagnus commutata – Silver Berry

061208 Weld county - Weber WPA_14 — Artemisia frigida – Pasture Sage

061208 Weld county - Weber WPA_15 — Cerastium arvense – Prairie Chickweed

070208_3 — Panicum capillare – Witchgrass

070208_10 — Unidentified ligule with long sheath

070208_12 — Long sheath and smooth ligule – unidentified

070208_16 — Sphaeralcea coccinea – Scarlet Globemallow

070208_17 — Toxicodendron radicans – Poison Ivy

070208_21 — Another shot of the very hair, hairy ligule and leaf of this unidentified grass sp

I wish I could identify these grass species where I just have ligule pictures, but it’s nearly impossible without seeing the actual plant. I didn’t take enough pictures to identify it that way.

070208_27 — Carex ligule from another angle

070208_30 — Amorpha canescens – Leadplant inflorescence

070208_31 — Amorpha foliage characteristics, part of the Fabaceae family (characteristic compound leaf structure)

070208_35 — Lytta nuttalli – Nuttal Blister Beetle

070208_36 — Cypripedium candidum – Lady Slipper Foliage

070208_39 — Cypripedium candidum – Rare Lady Slipper on the North Dakota Prairie

070208_44 — Great shot of a rare prairie lady slipper

070208_51 — Wheatgrass sp based on clasping auricle presence – possibly quackgrass

blanketflower2 — Gaillardia aristata – Blanketflower

070208_54 — Unidentified hairy ligule with hairy leaf margins

Asclepias sp, probably incarnata – pink/swamp milkweed

Asclepias spp. Probably sullivantii – Prairie Milkweed

Penstemon digitalis or gracilis – Beardtongue

Lilium philadelphicum – Wood Lily, or at least a Lily species

Ratibida columnifera – Prairie coneflower

Echinacea angustifolia – Purple Coneflower – Echinacea

Andropogon geradii – Big Bluestem – Turkey foot looking inforescence

Andropogon geradii – Big Bluestem ligule

Schizachyrium scoparium – little bluestem

This was just a short list of the plants I saw and categorized. It’s unfortunate that I didn’t classify these plants shortly after I took the pictures. The grasses are hard to identify without having the whole plant in front of me, but during the survey period, I knew them perfectly. I wish I would have continued to create a ligule guide with key to the North Dakota prairie. The prairie there is a mix of short and tall grass, with hundreds of native grass species present, with nearly all of them being bunch grasses. Maybe I will get the opportunity again to classify the grasses and forbs of ND.

Skills

Seining

01/17/2016 Eric Hanson Leave a comment

One of the best pieces of survey equipment I’ve used is the seine. I enjoy it so much because even the most dull stream is full of aquatic invertebrates and small fishes. The piece of equipment is composed of two long poles with a net strung between them. On the top side of the net there were floats, and on the bottom were weights.

Seining for a research project at MSUM — Seining at the Buffalo River Science Center for a research project at MSUM

When I was working on creating a photographic guide to the fishes of the Buffalo River, a dear professor and I went out and seined many streams and ponds in the Buffalo River basin. If you have never tried it, it’s a great way to sample aquatic systems. It pulls up all of the macro invertebrates and the smallest fishes.

Skills

Laser Seed Counting

01/16/2016 Eric Hanson Leave a comment

One of the first jobs I had when I was still in school was working for the Agricultural Research Service (ARS) at the Northern Great Plains Research Laboratory in Mandan, ND. They were a USDA affiliated lab, and I worked with a soil scientist on a crop rotation program. The project had gone on for quite some time, and I spent most of my days there on test plots making sure everything was going smoothly.

We started in spring by drilling in seeds, created quadrants, fertilized and tended those quadrants, and then harvested them in the fall. Since everything was laid out in a grid and we had information going back for years on everything including: soil moisture, nutrient/fertilizer loads, plant seed chemical analysis, and pesticide and herbicide use. This information was essential in creating a guide to which crops to plant for successive years. The office even produced a computer program for farmers to plan their fields out for years in advance.

After each quadrant was harvested, we used a shaker machine to separate the chaff, and then a laser seed counter to count how many seeds were produced. Those seeds then went to a grinder and then shipped out for analysis. It was so long ago that I did this work, Don Tanaka or Justin Hartel may be a better resource for questions.

One of my primary functions was bagging, counting, and organizing the seeds after they came off the sieve/shaker machine. The laser counters had to be calibrated and tweaked to get the most accurate count. So I spent quite a bit of time counting out 100 seeds and then running them through the machine to see how accurate we could get it. Weight wasn’t a reliable number due to each plant having different sized seeds.

Learning Through Life